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    Characterization and analysis of the exogenous application of selected phytohormones on C. reinhardtii metabolism

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    Genes to Fuels.pdf (25.43Mb)
    No. of downloads: 659

    Date
    2011
    Author
    Chang, Andrew
    Chang, Maria
    Feng, Patrick
    Khural, Jasjeet
    Luo, Tana
    McCarthy, James
    Mekelburg, Cory
    Nadig, Kelsey
    Perry, Christine
    Thaper, Sharad
    Urbanski, Rick
    Vohra, Pragun
    Weber, Christian
    Wong, Justin
    Advisor
    Kahn, Jason D.
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    Abstract
    Introduction of algal biofuel on an industrial scale will only be possible if production cost can be lowered, either by speeding algal growth, increasing lipid production per cell, or both. Our approach examined the application of phytohormones to algae to reach this goal. Bioinformatics and literature led us to four phytohormones: Indole-3-acetic acid, Epibrassinolide, Trans-zeatin, and Trans-zeatin-riboside. These were systematically introduced to algae at a range of concentrations. IAA, Epibrassinolide, and Trans-zeatin-riboside increased algae growth rates at concentrations of 10-8M to 10-12M. A treatment of Trans-zeatin-riboside yielded an increase in growth rate of 31.1 ± 9.4% (p < 0.05, n=8), an increase in algal dry mass by 38.9 ± 11.9% (p < 0.05, n=8), a negligable change in specific lipid mass (p < 0.05, n=8), and, most notably, a lipid concentration increase of 44.6 ± 42.1% (p < 0.05, n=8). A treatment of Trans-zeatin-riboside at 10-11 M yields practical benefits to biodiesel production.
    Notes
    Gemstone Team Genes to Fuels
     
    2011 Recipient of the Library Award for Undergraduate Research
    URI
    http://hdl.handle.net/1903/11393
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    • Gemstone Team Research

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    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility