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Metabolic Profiling of in vitro Cultured Medicinal Plants: Actaea racemosa L

dc.contributor.advisorBrosi, Sunshine
dc.contributor.authorMassimino, Christopher
dc.identifier.citationThe University of Maryland McNair Scholars Undergraduate Research Journal, 2, (2010): 157-165.en_US
dc.description.abstractThe use of Actaea racemosa L., black cohosh, as an herbal supplement has dramatically increased over the past decade. The rhizome is used for a variety of medicinal purposes involving premenopausal symptoms. This project involved the development of a protocol for establishment of in vitro callus cultures from excised tissues of racemes and leaves. Explants were grown on Murashige and Skoog medium (MS) with a variety of concentrations of growth hormones TDZ and NAA. The production of secondary metabolites by these callus cultures is being analyzed by ultra high pressure liquid chromatography coupled with mass spectrometry UHPLC/MS. The first inoculation of racemes was observed after one week with a large growth of fungi in twelve of the sixteen plates. The project is ongoing and awaiting further results of callus growth in the plates.en_US
dc.subjectActaea racemosa Len_US
dc.subjectBlack cohoshen_US
dc.titleMetabolic Profiling of in vitro Cultured Medicinal Plants: Actaea racemosa Len_US
dc.relation.isAvailableAtRonald E. McNair Post-Baccalaureate Degree Program
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtUniversity of Maryland (College Park, Md)

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