Library Faculty/Staff Scholarship and Research

Permanent URI for this collectionhttp://hdl.handle.net/1903/11

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Author: search.filters.author.Sprecher, HowardSubject: search.filters.subject.Baykoucheva

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    Pathways for the biosynthesis of polyunsaturated fatty acids
    (1996) Sprecher, Howard; Luthria, Devanand; Baykousheva, Svetla P.; Mohammed, Selma B.
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    Peroxisomal-microsomal communication in unsaturated fatty acid metabolism
    (Elsevier, 1995) Baykousheva, Svetla P.; Luthria, Devanand L.; Sprecher, Howard
    The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by β-oxidation of [1-14C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity was due to microsomal contamination. The production of acid-soluble radioactivity from [1-14C]7,10,13,16–22:4, but not from [3-14C]7,10,13,16–22:4 was independent of 1-acyl-GPC, with and without microsomes. By comparing rates of peroxisomal β-oxidation with those for microsomal acylation, it was shown that the preferred metabolic fate of arachidonate, when added directly to incubations, or generated via β-oxidation, was esterification by microsomal 1-acyl-GPC acyltransferase, rather than continued peroxisomal β-oxidation.
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    The role played by β-oxidation in unsaturated fatty acid biosynthesis
    (1994) Sprecher, Howard; Baykousheva, Svetla
    The authors hypothesized that a role for peroxisomes might be to chain shorten fatty acids but that the products would be transported to the endoplasmic reticulum where they would be esterified into lipids. Results showed that when microsomes and 1-acyl-sn-glycero-3-phosphocholine are added to peroxisomes that competing reactions take place, i.e. β-oxidn. and esterification.