UMD Theses and Dissertations

Permanent URI for this collectionhttp://hdl.handle.net/1903/3

New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a given thesis/dissertation in DRUM.

More information is available at Theses and Dissertations at University of Maryland Libraries.

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2015 - 20162

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Subject: search.filters.subject.Endocrine Disruption

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    A NOVEL NON-LETHAL LAPAROSCOPIC APPROACH TO DETECT INTERSEX (TESTICULAR OOCYTES) IN LARGEMOUTH BASS (MICROPTERUS SALMOIDES) AND SMALLMOUTH BASS (MICROPTERUS DOLOMIEU)
    (2016) MacLeod, Alexander Hugh; YONKOS, LANCE T; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    The appearance of testicular oocytes (TO) in wild fish populations has received considerable attention in the scientific literature and public media. Current methods to quantify TO are lethal; instead, a non-lethal alternative was examined. Laparoscopic insertion into the genital pore allowed internal visualization of the gonad and detection of TO by collecting five testis biopsies in smallmouth bass Micropterus dolomieu and largemouth bass Micropterus salmoides. Overall, biopsies quantified similar levels of TO detection and severity to conventional transverse sectioning with less than 10% mortality. Suitability of surgical anesthetics, tricaine methanesulfonate and electronarcosis were examined in laboratory and field applications. Electronarcosis had the added benefit of rapid sex identification and immediate release of female fish with minimal trauma, representing significant benefits when sampling small or compromised populations. Laparoscopy may be useful for monitoring the prevalence and severity of TO in these fish species when lethal sampling is not a desired outcome.
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    INVESTIGATING COMPOSTING AS A METHOD FOR REDUCING ESTROGENICTY IN POULTRY LITTER AND BIOSOLIDS
    (2015) Hammett, Kirsten; Yonkos, Lance; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Biosolids and poultry litter contain the natural estrogens 17β-estradiol and estrone, which can be transported to receiving waters via runoff when these materials are used as fertilizers. Estrogens are of concern because of their ability to act as endocrine disruptors and feminize fish. In this study, In-Vessel Aerated and Turned composting was investigated for its efficacy at mitigating estrogen concerns in BS and PL. Pre- and post- composted, BS and PL samples were investigated for total estrogenicity and estrogen species concentrations. In addition, conversion of estrone to 17β-estradiol was investigated by measuring the creation of deuterated 17β-estradiol from a deuterium-labeled estrone stock within aqueous PL mixtures. Data from these studies indicates that there may be efficacy in composting BS and PL prior to land application and suggest that estrone is capable of converting to the more potent 17β-estradiol species as a result of entering microbially active environments.