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dc.contributor.advisorChen, Yuen_US
dc.contributor.authorTang, Qinggong Tangen_US
dc.date.accessioned2017-09-13T05:39:39Z
dc.date.available2017-09-13T05:39:39Z
dc.date.issued2017en_US
dc.identifierdoi:10.13016/M25T3G07S
dc.identifier.urihttp://hdl.handle.net/1903/19841
dc.description.abstractLaminar optical tomography (LOT) is a mesoscopic three-dimensional (3D) optical imaging technique that can achieve both a resolution of 100-200 µm and a penetration depth of 2-3 mm based either on absorption or fluorescence contrast. Fluorescence laminar optical tomography (FLOT) can also provide large field-of-view (FOV) and high acquisition speed. All of these advantages make FLOT suitable for 3D depth-resolved imaging in tissue engineering, neuroscience, and oncology. In this study, by incorporating the high-dynamic-range (HDR) method widely used in digital cameras, we presented the HDR-FLOT. HDR-FLOT can moderate the limited dynamic range of the charge-coupled device-based system in FLOT and thus increase penetration depth and improve the ability to image fluorescent samples with a large concentration difference. For functional mapping of brain activities, we applied FLOT to record 3D neural activities evoked in the whisker system of mice by deflection of a single whisker in vivo. We utilized FLOT to investigate the cell viability, migration, and bone mineralization within bone tissue engineering scaffolds in situ, which allows depth-resolved molecular characterization of engineered tissues in 3D. Moreover, we investigated the feasibility of the multi-modal optical imaging approach including high-resolution optical coherence tomography (OCT) and high-sensitivity FLOT for structural and molecular imaging of colon tumors, which has demonstrated more accurate diagnosis with 88.23% (82.35%) for sensitivity (specificity) compared to either modality alone. We further applied the multi-modal imaging system to monitor the drug distribution and therapeutic effects during and after Photo-immunotherapy (PIT) in situ and in vivo, which is a novel low-side-effect targeted cancer therapy. A minimally-invasive two-channel fluorescence fiber bundle imaging system and a two-photon microscopy system combined with a micro-prism were also developed to verify the results.en_US
dc.language.isoenen_US
dc.titleNOVEL TECHNOLOGIES AND APPLICATIONS FOR FLUORESCENT LAMINAR OPTICAL TOMOGRAPHYen_US
dc.typeDissertationen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentBioengineeringen_US
dc.subject.pqcontrolledBiomedical engineeringen_US
dc.subject.pqcontrolledEngineeringen_US
dc.subject.pqcontrolledOpticsen_US
dc.subject.pquncontrolledBone tissue engineeringen_US
dc.subject.pquncontrolledBrain functional imagingen_US
dc.subject.pquncontrolledCancer therapy monitoringen_US
dc.subject.pquncontrolledLaminar optical tomographyen_US
dc.subject.pquncontrolledMesoscopic imaging techniqueen_US
dc.subject.pquncontrolledMulti-modal optical imaging approachen_US


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