Objective: To examine the relationship between acculturation and BMI among Asian Americans. Methods: Data of 847 Chinese, Korean and Vietnamese recruited for a health education program in Maryland were included. Acculturation was measured by the short version of Suinn-Lew Asian Self-Identity Acculturation Scale (SL-ASIA) and its individual components. Height and weight were measured by trained staff. Multiple linear regression was used to estimate the parameters of acculturation variables. Results: After adjusting for confounders, SL-ASIA (β=0.71, 95% CI: 0.15, 1.26), having education in the U.S (β=0.56, 95% CI: 0.01, 1.11), younger age of arrival (0-5 years: β=3.32, 95% CI: 1.84, 4.80, 6-10 years: β=1.55, 95% CI: 0.02, 3.07) and equal preference of Asian/American food in restaurants (β=0.92, 95% CI 0.38, 1.46) were associated with BMI. The association between acculturation and BMI was stronger among men than women, and weakest among Vietnamese. Conclusion: Acculturation is moderately associated with BMI among Asian Americans.