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Please use this identifier to cite or link to this item: http://hdl.handle.net/1903/10484

Title: EVALUATION OF ANTIBIOTIC-RESISTANT BACTERIA IN TERTIARY TREATED WASTEWATER, RECLAIMED WASTEWATER USED FOR SPRAY IRRIGATION, AND RESULTING OCCUPATIONAL EXPOSURES
Authors: Goldstein, Rachel Elizabeth Rosenberg
Advisors: Sapkota, Amy R
Department/Program: Maryland Institute for Applied Environmental Health
Type: Thesis
Sponsors: Digital Repository at the University of Maryland
University of Maryland (College Park, Md.)
Subjects: Environmental Health
Health Sciences, Public Health
Health Sciences, Occupational Health and Safety
Keywords: antimicrobial resistance
methicillin-resistant Staphylococcus aureus
reclaimed wastewater
spray irrigation
vancomycin-resistant Enterococcus
wastewater
Issue Date: 2010
Abstract: Occupational exposures to vancomycin-resistant <italic>Enterococcus</italic> (VRE) and methicillin-resistant <italic>Staphylococcus aureus</italic> (MRSA) in reclaimed wastewater used for spray irrigation were evaluated. In 2009, wastewater was collected from a tertiary-treatment facility, and reclaimed wastewater, nasal and dermal swab samples from an irrigation site. Samples were evaluated for MRSA and VRE using standard methods, PCR, and susceptibility testing. MRSA and VRE were isolated from all wastewater samples except effluent. While wastewater MRSA isolates were multidrug resistant (98%), no MRSA was isolated in irrigation water or swabs. VRE was isolated in one irrigation water sample. Fewer irrigation workers were colonized with <italic>S. aureus</italic> (31%) compared to controls (46%), but they harbored more multidrug resistant <italic>S. aureus</italic>. This is the first study to 1) evaluate antibiotic-resistant bacteria (ARB) in U.S. reclaimed wastewater and resulting occupational exposures, and 2) detect MRSA in U.S. wastewater. The findings suggested that tertiary wastewater treatment effectively reduced MRSA and VRE.
URI: http://hdl.handle.net/1903/10484
Appears in Collections:Maryland Institute for Applied Environmental Health Theses and Dissertations
UMD Theses and Dissertations

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