http://hdl.handle.net/1903/15 2013-05-20T21:17:50Z 2013-05-20T21:17:50Z Hansen, John Norman Lieberman, Joshua A. http://hdl.handle.net/1903/9421 2009-08-28T02:35:01Z 2009-01-01T00:00:00Z

Title: Construction and Characterization of a Torsional Pendulum that Detects a Novel Form of Cranial Energy Authors: Hansen, John Norman; Lieberman, Joshua A. Abstract: A torsional pendulum consisting of a dome-shaped energy collector and a nylon monofilament support fiber was suspended above the cranium of a seated human subject and the effects of the subject on the oscillations of the pendulum were measured. There were dramatic effects, with FFT analysis of the oscillation signal showing many new frequencies in addition to the natural frequency of 0.034 Hz. The lowest new frequencies (0.0-0.002 Hz) were accompanied by a shift in the Center of Oscillation (COO) of the pendulum, and the higher frequencies were associated with changes in the amplitude of oscillation. The Delta COO (7.3 deg) and the amplitude (12 deg) effects were substantial, and would require forces equivalent to 34 and 56 mg, respectively. Residual effects on the Delta COO and amplitudes persisted for at least 30 min after the subject departed, and the rate at which they subsided conformed to the kinetics of a chemical relaxation process with a relaxation time of 600 sec. Shifts in the magnitude of the Delta COO with the subject present also conformed to chemical relaxations processes, with relaxation times of 35 and 200 sec. It is proposed that the energy that drives the anomalous oscillations when the subject is present is the result of enzyme-mediated energy transductions that convert metabolic energy into a form of energy that can affect the pendulum. Although highly speculative, it is suggested that aspects of quantum entanglement are involved in the energy transduction process.

2009-01-01T00:00:00Z Ma, Ling Melegari, Monica Colombini, Marco Davis, Jeffery http://hdl.handle.net/1903/8295 2008-08-06T02:34:04Z 2008-02-15T00:00:00Z

Title: Large and Stable Transmembrane Pores from Guanosine-Bile Acid Conjugates Authors: Ma, Ling; Melegari, Monica; Colombini, Marco; Davis, Jeffery

2008-02-15T00:00:00Z Kahn, Jason D. Cheong, Raymond Edelman, Laurence M. Mehta, Ruchi A. Morgan, Michael A. http://hdl.handle.net/1903/3959 2007-12-01T05:17:32Z 2006-10-01T00:00:00Z

Title: Flexibility and Control of Protein-DNA Loops Authors: Kahn, Jason D.; Cheong, Raymond; Edelman, Laurence M.; Mehta, Ruchi A.; Morgan, Michael A. Abstract: Protein-DNA loops are essential for efficient transcriptional repression and activation. The geometry and stability of the archetypal Lac repressor tetramer (LacI)-DNA loop were investigated using designed hyperstable loops containing lac operators bracketing a sequence-directed bend. Electrophoretic mobility shift assays, DNA cyclization, and bulk and single-molecule fluorescence resonance energy transfer (FRET) demonstrate that the DNA sequence controls whether the LacI-DNA loop forms a compact loop with positive writhe or an open loop with little writhe. Monte Carlo methods for simulation of DNA ring closure were extended to DNA loops, including treatment of variable protein hinge angles. The observed distribution of topoisomer products upon cyclization provides a strong constraint on possible models. The experiments and modeling imply that LacI-DNA can adopt a wide range of geometries but has a strong intrinsic preference for an open form. The flexibility of LacI helps explain in vivo observations that DNA looping is less sensitive to DNA length and shape than would be expected from the physical properties of DNA. While DNA cyclization suggests two pools of precursor loops for the 9C14 construct, single-molecule FRET demonstrates a single population. This discrepancy suggests that the LacI-DNA structure is strongly influenced by flanking DNA. Description: Combined research report and review article on our work in DNA looping. Presented at the Asia and Pacific Workshop on Biological Physics, Singapore, July 2006.

2006-10-01T00:00:00Z